Determination of total and esterified cholesterol in serum.
نویسندگان
چکیده
A simple method for the determination of total and esterified cholesterol is described. Serum lipids are extracted into a solvent mixture and aliquots of the extract are analyzed directly for free and total cholesterol by the Zlatkis reaction. Esterified cholesterol is calculated by difference. Free cholesterol is determined under conditions of temperature and solvent composition such that the cholesterol esters do not react. The method yields quantitative recoveries of free and esterified cholesterol from serum. Data are presented on the precision of the method, and on results of a comparison with the reference procedure of Sperry and Webb.
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عنوان ژورنال:
- Clinical chemistry
دوره 12 11 شماره
صفحات -
تاریخ انتشار 1966